western blot stripping buffer - please recomend a stripping buffer for re-bloting (May/04/2009 )
Could anyone recommend a stripping buffer for re-bloting western blot membranes? Thanks.
-apple9-
I use the following:
100mM 2-mercapto ethanol
2% SDS
62.5 mM Tris HCl PH6.7
-Guillermina-
If it is PVDF membrane i use 0.2 N NaoH.
Its so convinient and time also less.
-newarray-