Thermal stability of H202 - stability of H2O2 in hot agar (May/04/2009 )
Hi everyone,
I just needed to know the best way to add hydrogen peroxide in an agar. I understand H202 can't be autoclaved. So I need to add it after the agar cools down. But everytime I do it, I am not sure if I am adding it at the right temperature so as not to degrade the H202. Anyone know if I can add H202 when the temperature is around 70 degree celsius which is when I usually add it. If I go to ard 60 degree celsius, then I have to be very quick otherwise the agar solidifies. Any suggestions would be appreciable.
Regards,
rigien
-rhythmgenes-
Don't have much experience with this but why can't you just spread it on top of agar when it cools down?
rhythmgenes on May 4 2009, 01:41 PM said:
Hi everyone,
I just needed to know the best way to add hydrogen peroxide in an agar. I understand H202 can't be autoclaved. So I need to add it after the agar cools down. But everytime I do it, I am not sure if I am adding it at the right temperature so as not to degrade the H202. Anyone know if I can add H202 when the temperature is around 70 degree celsius which is when I usually add it. If I go to ard 60 degree celsius, then I have to be very quick otherwise the agar solidifies. Any suggestions would be appreciable.
Regards,
rigien
I just needed to know the best way to add hydrogen peroxide in an agar. I understand H202 can't be autoclaved. So I need to add it after the agar cools down. But everytime I do it, I am not sure if I am adding it at the right temperature so as not to degrade the H202. Anyone know if I can add H202 when the temperature is around 70 degree celsius which is when I usually add it. If I go to ard 60 degree celsius, then I have to be very quick otherwise the agar solidifies. Any suggestions would be appreciable.
Regards,
rigien
-T C-