Culturing QT6 cell line - Ideal culture conditions (Apr/27/2009 )

I'm currently trying to culture QT6 cells and am having much difficulty.

First my culture media:
Medium 199 with Earle's salts
5% FBS
10% tryptose phosphate broth
1% DMSO
+ antibiotic (pen/strep)

I tried this medium to begin with but my cell started dying. They look spindly & there are a lot of floaters.

Then I took away the DMSO and the cells did a bit better.

I was wondering if anyone could give me advice on culturing these cells.

Thanks!

-andreanyu-

Even 0.1% DMSO will kill your cells quite quickly unless they are frozen.

The ATCC recommends:

ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Temperature: 37.0°C

Also, if you really can't get the cells to grow, you can try fiddling with the CO₂ concentration. I used to work with a cell line that was recommended for growth at 5%, but I got much better growth at 10%.