Protocol for 16S rDNA real time PCR - (Apr/27/2009 )

Hi everyone...

I am very new in this field of molecular biology....I have been asked to detect bacteria in chicken ileum using the 16S rDNA real time PCR technique...Does anyone have a protocol for that? or any good paper?
plse help me...i'm near to tears!

-nee-

nee on Apr 27 2009, 11:47 PM said:

Hey there,
you can try to read papers like this: http://jmm.sgmjournals.org/cgi/content/abstract/52/8/685
protocols are usuallly modified for your own samples and pcr reagents. good luck!!

-chrisbelle-

Thank you so much for your help

-nee-