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Selecting for Streptococcus pneumoniae against Enterococci - (Apr/17/2009 )

Hi all

I am trying to plate some Strep pneumo from a culture which is composed mostly of Enterococci. Sources say that adding gentamicin can select for Strep pneumo however I am not sure if this does anything to Enterococci. Is there a way where these two groups of the same species can be separated?

Thanks so much

LabNerd

-LabNerd-

LabNerd on Apr 17 2009, 04:06 AM said:

Hi all

I am trying to plate some Strep pneumo from a culture which is composed mostly of Enterococci. Sources say that adding gentamicin can select for Strep pneumo however I am not sure if this does anything to Enterococci. Is there a way where these two groups of the same species can be separated?

Thanks so much

LabNerd


If you have sheep blood agar plates, you should be able to differentiate based on hemolysis type. S. pneumo is alpha hemolytic, and Enterococcus is generally gamma hemolytic.

-gfischer-

They're not the same species.

I think neomycin muight work - but recall some of the enterococci (vancomycin resistant?) were also resistant to neomycin.

-GeorgeWolff-