1.5% agar plate - (Apr/13/2009 )
Im an amateur researcher and I just got into the mucrobiology field. Before this I was doing animal science. I hope to learn more about the basics and principles of microbiology. I have been trying to figure this out but couldnt get any information on the net. They didnt explain why..
I would like to know what is the reason behind researchers using 1.5% agar plate? What is the significance between 1.5% and other percentages? If we use 1% is there any difference?
This is strictly a matter of taste and convenience. 1% agar is a bit soft and is easy to tear with a loop. 1.5% agar has a harder surface. The choice may slightly change the morphology of colonies, so if this is an important phenotypic characteristic, it might matter. 1.5% is the "normal" choice.
phage434 on Apr 13 2009, 05:09 AM said:
so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..
c0ok1e on Apr 13 2009, 02:44 PM said:
phage434 on Apr 13 2009, 05:09 AM said:
so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..
you could find some papers on the subject.
Like:
1 or2 or 3 but how they got the 1.5% ? No idea, I guess trail and error? Someone found out that it was good to use 1.5% and well since then... I have no idea of an article is written on why 1.5%.. but maybe you could find some references in the texts I gave that refer to some older literature that speak about the 1.5% agar.
pito on Apr 13 2009, 08:56 AM said:
c0ok1e on Apr 13 2009, 02:44 PM said:
phage434 on Apr 13 2009, 05:09 AM said:
so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..
you could find some papers on the subject.
Like:
1 or2 or 3 but how they got the 1.5% ? No idea, I guess trail and error? Someone found out that it was good to use 1.5% and well since then... I have no idea of an article is written on why 1.5%.. but maybe you could find some references in the texts I gave that refer to some older literature that speak about the 1.5% agar.
ALright thanks.. Ill look into it... Maybe its not so important after all but I just like to know my work in detail...
Some of the commercial powdered-agar media come in 1.2% of agar, but we prefer to use harder agar so we add additional agar powder when preparing.
amateur researcher = terrorist
housemusicanl on May 12 2009, 02:46 PM said:
An agar terrorist??? Deciding factor on agar percentages...........budget!!!!
We're not allowed to ask questions of terrorists with reading their Miranda rights 1st.
It's well known that Real Terrorists (tm) use sliced boiled potatoes as culture medium.
Nothing to worry about here.