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1.5% agar plate - (Apr/13/2009 )

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Im an amateur researcher and I just got into the mucrobiology field. Before this I was doing animal science. I hope to learn more about the basics and principles of microbiology. I have been trying to figure this out but couldnt get any information on the net. They didnt explain why..

I would like to know what is the reason behind researchers using 1.5% agar plate? What is the significance between 1.5% and other percentages? If we use 1% is there any difference?

-c0ok1e-

This is strictly a matter of taste and convenience. 1% agar is a bit soft and is easy to tear with a loop. 1.5% agar has a harder surface. The choice may slightly change the morphology of colonies, so if this is an important phenotypic characteristic, it might matter. 1.5% is the "normal" choice.

-phage434-

phage434 on Apr 13 2009, 05:09 AM said:

This is strictly a matter of taste and convenience. 1% agar is a bit soft and is easy to tear with a loop. 1.5% agar has a harder surface. The choice may slightly change the morphology of colonies, so if this is an important phenotypic characteristic, it might matter. 1.5% is the "normal" choice.


so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..

-c0ok1e-

c0ok1e on Apr 13 2009, 02:44 PM said:

phage434 on Apr 13 2009, 05:09 AM said:

This is strictly a matter of taste and convenience. 1% agar is a bit soft and is easy to tear with a loop. 1.5% agar has a harder surface. The choice may slightly change the morphology of colonies, so if this is an important phenotypic characteristic, it might matter. 1.5% is the "normal" choice.


so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..


you could find some papers on the subject.
Like:
1 or2 or 3 but how they got the 1.5% ? No idea, I guess trail and error? Someone found out that it was good to use 1.5% and well since then... I have no idea of an article is written on why 1.5%.. but maybe you could find some references in the texts I gave that refer to some older literature that speak about the 1.5% agar.

-pito-

pito on Apr 13 2009, 08:56 AM said:

c0ok1e on Apr 13 2009, 02:44 PM said:

phage434 on Apr 13 2009, 05:09 AM said:

This is strictly a matter of taste and convenience. 1% agar is a bit soft and is easy to tear with a loop. 1.5% agar has a harder surface. The choice may slightly change the morphology of colonies, so if this is an important phenotypic characteristic, it might matter. 1.5% is the "normal" choice.


so there are no published paper on the different percentage of agar used?
Then it means that the hardness of agar varies from 1 - 1.5% are not so important right.. thanks..


you could find some papers on the subject.
Like:
1 or2 or 3 but how they got the 1.5% ? No idea, I guess trail and error? Someone found out that it was good to use 1.5% and well since then... I have no idea of an article is written on why 1.5%.. but maybe you could find some references in the texts I gave that refer to some older literature that speak about the 1.5% agar.


ALright thanks.. Ill look into it... Maybe its not so important after all but I just like to know my work in detail...

-c0ok1e-

Some of the commercial powdered-agar media come in 1.2% of agar, but we prefer to use harder agar so we add additional agar powder when preparing.

-why-

amateur researcher = terrorist

-housemusicanl-

housemusicanl on May 12 2009, 02:46 PM said:

amateur researcher = terrorist


An agar terrorist??? Deciding factor on agar percentages...........budget!!!!

-Jaff-

We're not allowed to ask questions of terrorists with reading their Miranda rights 1st.

-GeorgeWolff-

It's well known that Real Terrorists (tm) use sliced boiled potatoes as culture medium.
Nothing to worry about here.

-phage434-
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