wrong gene - (Apr/11/2009 )
give it a blast and what did u get?
what gene is the most similar to urs?
if u could translate it into protein that would be even better.
do an ORF find and then blast
blast result:
highest similarity: 82% - Enterobacter sp. polysaccharide deacetylase domain protein
my working bacteria is Klebsiella pneumoniae. so if i choose K. pneumoniae
similarity: 77% - Klebsiella pneumoniae 342 - polysaccharide deacetylase domain protein
for ur reference, i paste my sequence result here (FASTA format):
>TrC-G-2-T7
NNNTNNCNAGTCCATGCTCCGGCCGCCATGGCGGCCGCGGGAATTCGATTACCATCGGCG
GCACCTGCGTTACCCCATTCTGAATAAGCTCAAAGACCGTCTGAATCAGACGTGGTATCA
AATCCGCATTGGCAATCGCCTGGCGTGGATCAGCAGCCTGGATGCGCAGGAAGATAACGG
CATCCCGGTTCTCACCTACCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCA
TACCTCCACGACCACCAGCGTACGTGCGTTCAGCAACCAGATGACCTGGCTTCGCGATCA
GGGATACGCCACGCTGACGATGTACCAGCTGGAAGGCTACGTGCGTAACAAGATGAACCT
GCCCGCGAAGGCGGTGGTGATTACGTTTGACGATGGGCTGAAGTCGGTGAGCCGTTACGC
TTACCCGGTTCTGAAAGAATATGGCTTCAACGCGACGGCGTTTATCATTTCGTCACGCAT
CAAAGGTCATCCGCAGAAGTGGGACCCAAAATCGCTGCAGTTTATGAGCGTTCAGGAAAT
TAAGGGCATACAGGACGTGTTCGACATTCAGTCTCACACTCACTTCCTGCATCGCGTGGA
TGGGTATAAACACCCGATTTTATTGAGCCGCAGCTATCACGTGATCCTGTTCGATTTTGA
ACGTTCCCGCCGTGCGCTGTCGCAGTTCAACCCGCGCGTGCTGTACCTTTCTTACCCGTT
TGGCGGCTATGACAATAAAGCGATCAAGGCAGCGAACGACGCCGGTTTTCATCTGGCGGT
AACGACGGTGAAAGGGAAGGTCAAGCCGGGGGATAATCCGTTCTTACTGAAACGCCTCTA
TATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGATCAGCAATCAGCCGCAGGG
GTAGGATTAATTGTAGGAGATGGTGAACACGCCGGAAGCGCTAAACGCGCCGGGGGTAAT
CATGCTGCCATCTGCGATCAGTTGGGCCACAAAGTTATCTGCGTCGACGTGGGACACTGT
TTCGTCATGTCATACGACGAGTCGCATCATGGGATAGCTGCAGTCCCGTGTATACAGATC
TCACGCCCGNACTGCGCCCTGTGANCGTATCGCNANAGTCATGNCTATNCCGTCANNNNN
TCTGANCTATGNATATCNNCCNCGCAGNGCCGCCNATGTATCNCT
the seq of primers that u used to amplify the gene and the paper associated with it. attach it.
we are close to solving ur puzzle
Primer A1 ACCATCGGCGGCACCTGCGT 20-mer
(Forward)
Primer A5 ACCATCGTCAGGTAGGGGACCAA 23-mer
(Reverse)
EXPECTED SIZE = 1238bp
here by i attach u the journal published.
