Liquid and solid autoclave - (Mar/31/2009 )
mdfenko on Apr 3 2009, 10:09 PM said:
the only problem with autoclaving solids (dry) with liquid cycle is that the wrappers sometimes came out damp or wet.
Really?
I havent got any setting to be decided. However you can indeed decide how fast you let the pressure go by turning the vial less or more open, but still, thats not really a specific setting.
Like said: on and off button and thats it
How would you guys keep your tips for the pipettes dry when autoclaving them.
We put aluminum foil around the boxes, but I think this is a waste of foil.
Arent there any alternatives?
josse on Aug 28 2009, 07:25 AM said:
We put aluminum foil around the boxes, but I think this is a waste of foil.
Arent there any alternatives?
Hi Josse,
Yes, that's a waste of foil. Just put the tips in a glassware oven after autoclaving, and they'll dry.
regards,
lab rat
lab rat on Aug 28 2009, 07:08 PM said:
josse on Aug 28 2009, 07:25 AM said:
We put aluminum foil around the boxes, but I think this is a waste of foil.
Arent there any alternatives?
Hi Josse,
Yes, that's a waste of foil. Just put the tips in a glassware oven after autoclaving, and they'll dry.
regards,
lab rat
I have done it with aluminum foil too: if you do not use aluminum foil, they stay wet for too long.
It is indeed a waste.
But arent there special bags to use instead of aluminim foil?
lab rat on Aug 28 2009, 09:08 AM said:
josse on Aug 28 2009, 07:25 AM said:
We put aluminum foil around the boxes, but I think this is a waste of foil.
Arent there any alternatives?
Hi Josse,
Yes, that's a waste of foil. Just put the tips in a glassware oven after autoclaving, and they'll dry.
regards,
lab rat
In my former department, we placed our wet autoclaved tip boxes into a heated drying cabinet. It took a while but the boxes come out dry the next morning
I know, we have that system too, but it is costly.
On a similar note, my DEPC-H2O protocol requires a sterilization time of 15min per 1L of water. Why is the increase in sterilization time necessary?
Just a general notice:
Why are you autoclaving tips? To clean them?
In my lab, like thousand other labs, we have only one autoclave used for tips and cell garbages. Sometime it happen that bags containing garbages open, and then everything in the autoclave is contaminated. And I am wonder for next cycles too.
Personal opinion, I prefer close safely tips bags and don' t autoclave tips.
You can autoclave them together.
Our lab has a very old autoclave, similar to yours, which only has an on/off switch and timer setting. We use 20min for solids only and 25min for liquids (or solids + liquids).
Tips must be sterile when working with bacteria, for example, which is why they are autoclaved -- to avoid any potential contamination.