what gram negative coc? - (Mar/28/2009 )
GeorgeWolff on Apr 3 2009, 03:06 AM said:
Think klebsiella may be your bug.
note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.
note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.
Are you sure about that? In literature they refer to it as no production.
-pito-
another feature of klebsiella is they are quite slimy. most klebsiella i have seen are like that.
-hanming86-
Yes pito - the literature for differentiation refers to a technique that doesn't detect lesser amounts. Put lead acetate paper in a tube of Klebsiella and it will demonstrate presence of H2S.
-GeorgeWolff-
GeorgeWolff on Apr 14 2009, 12:22 AM said:
Yes pito - the literature for differentiation refers to a technique that doesn't detect lesser amounts. Put lead acetate paper in a tube of Klebsiella and it will demonstrate presence of H2S.
Ah I see, interesting, I have indeed also found in some papers that they refer to "no production" at all when they really mean: a very small amount of H2S production. This is really misleading.
But you are refering to Klebsiella now as an organism that produces H2S, but do you also mean that ALL enterics (Enterobacteriaceae) produce H2S ?
You could be right.
Anyway, interesting topic , I have looked around a bit and it doenst seem easy to make a correct point on the matter (see attachments)
-pito-
a negative stain with India Ink should show you the presence/absence of a capsule?
-aimikins-
True but capsule is not a definitive character for Klebsiella.
-GeorgeWolff-
GeorgeWolff on Apr 3 2009, 03:06 AM said:
Think klebsiella may be your bug.
note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.
note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.
According to one of my teachers Shigella does not produce H2S.
-josse-
It doesn't appear to when one uses typical clinical trests. It does when you use lead citrate paper.
-GeorgeWolff-
GeorgeWolff on Apr 15 2009, 11:26 PM said:
It doesn't appear to when one uses typical clinical trests. It does when you use lead citrate paper.
Interesting, I am learning new things each day here.
You seem to know your microbiology GeorgeWolff, very handy to for us,novices, that you are here.
-pito-
GeorgeWolff on Apr 15 2009, 11:26 PM said:
It doesn't appear to when one uses typical clinical trests. It does when you use lead citrate paper.
GeorgeWolff, do you have some sort of reference about this? Some textbook or literature about this? I havent been able to find anything about this.
-josse-