Dulbecco's PBS (Ca++, Mg++ free) - Ask for recipe (Mar/13/2009 )

Dear all
could you please tell me how to prepare Dulbecco's PBS (Ca++, Mg++ free) ?? i need a recipe for preparing 1X solution in 1 Litre. Best regards.

it's a good question. I usually make my own PBS for normal lab usage, but for cell culture we use commercial mg- and ca-free PBS. I always wonder how they make it.

I buy 1xPBS from PAA. it's cheaper than SIGMA I guess.

Dulbecco's Phosphate Buffered Saline (PBSA) - Dissolve 0.2 g of potassium chloride, 0.2 g of monobasic potassium phosphate (KH2PO4), 8 g of sodium chloride, and 1.74 g of dibasic sodium phosphate (Na2HPO4.7H20) in 0.9 liter of distilled (or Milli Q) water. Adjust the pH to 7.2 with concentrated sodium hydroxide if needed. Make up to 1 liter with water. Sterilize by autoclaving.

Be carefully to note the water of crystallization of the dibasic sodium phosphate you use. If the anhydrous salt you will need only 1.14 g.

There are alternative recipes. These usually differ slightly n the concentration of phosphate.

I like BIOLOGICAL INDUSTRIES because they give the formulations for all their products.
This link has on the bottom the formulation for PBS x1 or x10 if you need it.

Note to all peoples that this is a PROTOCOLS site:

There is a page for protocols, linked right at the top of every page you visit. A quick search will find you what you need, especially for common solutions like PBS, Tris, etc.