DNA shearing with Misonix 4000 and microplates cup horn - (Mar/11/2009 )

Hi everybody,
I'm going crazy trying to shear the DNA of my Saos2 and MCF-7 with a Misonix 4000 sonicator with a microplate cup horn.
Any suggestion?
Please!!

What is your propblem? unable to shear DNA? what conditions are you using. Without any of these info, it is hard to troubleshoot.

there is a girl also doing DNA shearing with sonication. maybe you can have a communication.

but I want to know how to see or download the attatchment in the following site:

http://www.protocol-online.org/biology-for...14943more2.html