Good comprehensive resource for maintaining immortal cell lines? - (Mar/11/2009 )

Just looking for a reputable, preferably free, online resource to obtain information about protocols that support effective & responsible culturing/sub-culturing of immortal cell lines (i.e., J774s, etc.).

Thanks!

Pretty basic, but it is OK:
http://www.sigmaaldrich.com/life-science/c...hniques-12.html

Otherwise I recommend R.I Freshney's Culture of Animal Cells. It is the best reference material that I have come across.