Which cell line produce cytokine? - (Mar/06/2009 )

acute on Apr 15 2009, 03:58 PM said:

Good to hear that things are working.
You can use PHA-M at 10 ug/ml. Check with the manufacturers literature for optimal concentrations.
"1:100" PHA comes from the days when PHA preparations were very crude. These are not used today.

Hope this helps

klinmed on Apr 15 2009, 10:35 PM said:

Thanks again
But the problem is that the ug of my PHA-M (from gibco) is unknown. I searched the invitrogen website and didnot found anything. it is just PHA-M in the size of 10 ml.
think that I should use 1:100,right?

acute on Apr 15 2009, 09:19 PM said:

I have not used Gibco PHA for many years. Found this protocol in the black hole of my filing system:

Phytohemagglutinin (PHA-M): (Gibco #10576-015) lyophilized, Rehydrate vial with 10 ml water to make stock (100 µg/ml). Store aliquots at -20 oC. Use 0.05 ml in 5 ml culture.

I have not used Gibco PHA for many years. Found this protocol in the black hole of my filing system:

Phytohemagglutinin (PHA-M): (Gibco #10576-015) lyophilized, Rehydrate vial with 10 ml water to make stock (100 µg/ml). Store aliquots at -20 oC. Use 0.05 ml in 5 ml culture.