Explants used in direct somatic embryogenesis - (Mar/05/2009 )

Can we choose root as explant when performing direct somatic embryogenesis for plant tissue culture? What are the other forms of explants that we can use for direct somatic embryogenesis? Thanks... :)

Looks like homework to me... have you tried this link

bob1 on Mar 9 2009, 07:53 AM said:

I have tried to search from there so many times....But still cannot get the Confirmed answers...Anyways thanks for your reply...

Can anyone help me...

Looks like you need meristem for it work, so you could use parts of the roots, but then I haven't done it or studied it

bob1 on Mar 10 2009, 08:43 AM said:

Most of the journals that I have read are using cotyledons from seeds. If I directly used roots to perform the tissue culture, the techniques used seems to be organogenesis instead of direct somatic embryogenesis is it? I am confuse about it...

The roots, stem, leaf, meristem and other parts of plants can grow via direct somatic embryogenesis if the media and plant growth regulators used are right. However, you may need to try out which explants will give you the best result as different plants will give the different result.

Good luck

robbiebc on Mar 24 2009, 07:59 PM said:

I am now using the fully matured cotyledon parts from the zygotic embryo of the seed to perform the tissue culture. Thanks for your information. I appreciate it.