Plasmid replication - Plasmid replication (Mar/04/2009 )
yeah, but you have to think "exponentionally". if you start with one cancer cell you have two after 24h. if you start with one E.coli you would end up with ~4 x 10^21 after 24h (considering uninhibited growth). 
also if you have a high copy plasmid one E.coli will contain about 400 copies 
with one maxi prep you can yield up to 1 milligram plasmid DNA from one 100ml overnight culture.
I would never try to do plasmmid amplification in mammalian cells, always in E. coli. You get much more plasmid and I don't know a proper way to isolate plasmid from cell culture cells.
Stardust
tea-test on Mar 7 2009, 11:56 AM said:
also if you have a high copy plasmid one E.coli will contain about 400 copies
with one maxi prep you can yield up to 1 milligram plasmid DNA from one 100ml overnight culture.
do you know how plasmid mini or maxi-preps purify the plasmid of our interest and not the DNA of E.coli itself? does E.coli have its own plasmid? if yes then wouldn't that contaminate our transformed plasmid during purification? how to separate them from eachother?
sorry for my basic questions. i'm very new to cloning and stuff
concerning plasmid purification methods just look at the qiagen link I gave above. and no, the available E.coli strains don't have plasmids that would be copurified.