? definition of: thermodynamic stability at the 5' end - (Feb/26/2009 )
Dear All,
What is the definition of "thermodynamic stability at the 5' end" ? The passenger strand can be cleaved more easily close to its 5' end ? Is this a property of the passenger strand itself or does this depend strongly on the RNase proteins, pH, etc. around ?
Thanks
-thyme-
Hey
I am not sure but does it have something to do with the -OH group present at the 3' end, which is reactive and thus makes the 3' end thermodynamically less stable than the 5' end.
Take this with a word of caution.......I am guessing and am not sure 
Best
TC
-T C-
thyme on Feb 26 2009, 11:14 AM said:
Dear All,
What is the definition of "thermodynamic stability at the 5' end" ? The passenger strand can be cleaved more easily close to its 5' end ? Is this a property of the passenger strand itself or does this depend strongly on the RNase proteins, pH, etc. around ?
Thanks
What is the definition of "thermodynamic stability at the 5' end" ? The passenger strand can be cleaved more easily close to its 5' end ? Is this a property of the passenger strand itself or does this depend strongly on the RNase proteins, pH, etc. around ?
Thanks
After cleavage of dsRNA by Dicer, the RNA strand with less(or more?) stable base pairing at 5 ' end can be assembled into RISC complex. GC pairing is more stable than AT.
-WHR-