Dendritic_ cells - (Feb/23/2009 )

hi I am PhD student (Immunology) i am facing problem in lab when i isolat Monocyte from human blood using CD14 beads and generate them to dendritic cells most the cells die during incubation or lose them during the washing step, i need helo in solving that problem because i dont knoe the cause of that . Please

Regards

Hi inas,

could you tells us some more details. Actually, if you post your protocol we will be able to help you better.

Hi ,
My protocol is attached

Thank you for your reply

Please answer me as soon as possible, I realy need help
Regards