Maximal and minimal volume in T75 and T25 flask - (Feb/18/2009 )
Hi,
I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?
Thank you in advance
Biog on Feb 18 2009, 08:43 AM said:
I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?
Thank you in advance
For maximum - Check this protocol-online page - Maintenance of cell culture
For T-75 flask - maximum of 50 ml of medium.
For T-150 flask - maximum of 100 ml of medium.
For T-225 flask - maximum of 200 ml of medium.
Thank you very much, "cellcounter" 
!
All the best
Okay...here is the proper way to do it...
calculation is as follows;
0.2 to 0.5 x cm2
This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration
T75cm2 flask = 15-37.5mls
T150cm2 flask = 30-75mls
T185cm2 flask = 37-92.5mls
calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.
The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.
sciencedork on Mar 9 2009, 01:02 PM said:
calculation is as follows;
0.2 to 0.5 x cm2
This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration
T75cm2 flask = 15-37.5mls
T150cm2 flask = 30-75mls
T185cm2 flask = 37-92.5mls
calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.
The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.
OOOh ! I completely forget this !
Thanks a lot.
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