phenol kristallin preparation - (Feb/17/2009 )

hi
Iwould like to know how I have to prepare phenol kristallin for DNA extraction?please help me.tanks

-anahid-

Go to the protocols page and click on the "media and solutions" link, scroll down to the link that says "phenol equilibration" and click on the "cached" link (the actual site is down) and follow the instructions there.

# Phenol Equilibration (Marcantonio Lab)
http://home.earthlink.net/~erikyoung/protcls.html#...
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Be aware that phenol is quite toxic and is also a mild local anaesthetic so be very careful with large volumes.

-bob1-

Thanks bOb
this link is unavalibale.please tell me how can I equilibrate phenol and is it necessary to use hydroxyquinolin?

-anahid-

try this link: phenol search at vadlo

you can find a few methods.

-mdfenko-