How to wash ethanol away from tissue? - (Feb/13/2009 )

I am going to extract DNA from pieces of fish fins that have been stored in ethanol. I guess I have to wash away as much ethanol as possible before extraction but has anybody any good protocol for this?
Is it as simple as incubating in 1xPBS for an hour or two or do I have to go through a more complicated and extended washing protocol? Thanks in advance

-DocFlow-

If you pour off the liquid/supernatant and leave the fins to air dry in an eppendorf (in a laminar flow hood if you need sterility) the ethanol will evaporate pretty quickly, within about 30 mins i'd say if you remove most of the alcohol in the first step

-cotchy-

Hey

Just to make sure that all the ethanol goes off, may be I would go for repeated washing with 1X PBS and vortex at every stage. Three times should be more than enough.

Best
TC

-T C-

T C on Feb 13 2009, 01:55 PM said:

Hey

Just to make sure that all the ethanol goes off, may be I would go for repeated washing with 1X PBS and vortex at every stage. Three times should be more than enough.

Best
TC

Thanks to both of you. I guess I'll try with the PBS washing first, but simple air drying may work just as good. I'll have to try both.

-DocFlow-