glycine in unfolding buffer - (Feb/11/2009 )

Hi all,

I'm unfolding my protein in buffer containing 50mM phosphate, 50mM glycine, 8M urea, and 1mM DTT. Could anyone explain the role of glycine in this buffer?

Thanks!

the glycine is a buffer that extends the useful range of the phosphate buffer with overlapping buffering ranges.