MOCK INFECTED CELLS - (Feb/11/2009 )

Hi,

can anyone explain what Mock infected cells mean and give me some examples about materials to mock infection?

-bluebird-

It's when you infect cells with an empty vector. It's the control.

-little mouse-

little mouse on Feb 11 2009, 04:51 AM said:

It's when you infect cells with an empty vector. It's the control.

thanks yes it is for control but can you explain more. I'm new in this field. :blink:

-bluebird-

I guess that the infected cells are infected with a viral vector, to express a protein of interest, or to knockdown the expression of a protein. From this expression or knockdown of expression, you oberve a response. To be sure that the response is due to the expression of a protein, and not due to the infection itself, you infect control cells with the same viral vector but empty. It means it won't express the protein of interest.

-little mouse-

I asked the same question last week:

http://www.protocol-online.org/forums/inde...?showtopic=6168

-Curtis-