Cell count using a hemocytometer - (Feb/05/2009 )

Cell count using a hemocytometer

http://www.abcam.com/index.html?pageconfig...e&rid=11454

-Minnie Mouse-

This is different from the mallasez counting chamber , since the size of each square is 1mm .

-youyou-

I have a question?

I am doing sputum processing and when I finhsed from the haemocytometer count, i have to centrifuge the the filtrate of the sample and then remove the supernatant and leaving the cell pellet, I need to know how THEN resuspend the cell pellet in PBS and then adjust the cells/volume to give me 0.5 million for preparation of cytospin slides!

Thank you

-respirator-

Can you please give me the link of mallasez counting chamber.

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Solar Panels Australia

-addymichy-

This website provides an alternative approach to cell counting with a hemocytometer

http://www.ruf.rice.edu/~bioslabs/methods/microscopy/cellcounting.html

-unknownko-