1-butanol detection - (Feb/04/2009 )
Is there anyone had experience working with 1-butanol? I need to detect the very low concentration of 1-butanol (<1%) but HPLC, Gas chromatography can't be done in my lab. I also tried NAD+ assay but it's very difficult to detect butanol within this range. I really appreciate any idea or sharing experience!
Quasimondo on Feb 4 2009, 04:28 PM said:
You need something like this perhaps, i.e. gas detector tubes. Here is another company , and as attachment a specifications sheet. Have no experience with it but it may help.
hobglobin on Feb 5 2009, 02:19 AM said:
Quasimondo on Feb 4 2009, 04:28 PM said:
You need something like this perhaps, i.e. gas detector tubes. Here is another company , and as attachment a specifications sheet. Have no experience with it but it may help.
Thank you for your reply. This one is good for the detection of trace amount. However, the range of this detector is too narrow (5-100ppm). In my case, maybe it'll excess (1000-5000ppm)
. And also, it's for gas detection. In my case, I need to detect 1-butanol in media 
Quasimondo on Feb 5 2009, 02:56 AM said:
hobglobin on Feb 5 2009, 02:19 AM said:
Quasimondo on Feb 4 2009, 04:28 PM said:
You need something like this perhaps, i.e. gas detector tubes. Here is another company , and as attachment a specifications sheet. Have no experience with it but it may help.
Thank you for your reply. This one is good for the detection of trace amount. However, the range of this detector is too narrow (5-100ppm). In my case, maybe it'll excess (1000-5000ppm)
. And also, it's for gas detection. In my case, I need to detect 1-butanol in media But for HPLC or GC you would need also an extraction and dilution protocol, you cannot use the media directly. Something like a extraction or (vacuum) distillation with heat in a round bottom flask and then via a condenser collecting and separating the vapour in another flask. The 1-butanol fraction then can be used for further analysis (GC or one of these tubes). The amount of 1-butanol you can regulate via the amount of media in the flask or later using only a part of the fraction.
hobglobin on Feb 6 2009, 05:36 AM said:
Quasimondo on Feb 5 2009, 02:56 AM said:
hobglobin on Feb 5 2009, 02:19 AM said:
Quasimondo on Feb 4 2009, 04:28 PM said:
You need something like this perhaps, i.e. gas detector tubes. Here is another company , and as attachment a specifications sheet. Have no experience with it but it may help.
Thank you for your reply. This one is good for the detection of trace amount. However, the range of this detector is too narrow (5-100ppm). In my case, maybe it'll excess (1000-5000ppm)
. And also, it's for gas detection. In my case, I need to detect 1-butanol in media But for HPLC or GC you would need also an extraction and dilution protocol, you cannot use the media directly. Something like a extraction or (vacuum) distillation with heat in a round bottom flask and then via a condenser collecting and separating the vapour in another flask. The 1-butanol fraction then can be used for further analysis (GC or one of these tubes). The amount of 1-butanol you can regulate via the amount of media in the flask or later using only a part of the fraction.
Yes, I know that, but as I mentioned earlier, we have to sent out our samples for the detection by GC or HPLC, it takes time, and not convenient. Moreover, I need to screen many samples at the same time, so the high throughput method is needed. I searched for the long time and only the NAD+ assay by detection of NADH from the alcohol dehydrogenase reaction with butanol is applicable, but when we tried, it's not very sensitive and stable, so it's difficult to detect butanol.
Hey
What are you doing?
Is it a product of a reaction, if it is, you can put some tag on one of the reactants that will enable you to see the product, like use radioactive extender units in case of condensation reactions or use some fluorescence based technique. I detected fatty acids by using radioactive extender-malonyl CoA.
TC
T C on Feb 12 2009, 10:00 PM said:
What are you doing?
Is it a product of a reaction, if it is, you can put some tag on one of the reactants that will enable you to see the product, like use radioactive extender units in case of condensation reactions or use some fluorescence based technique. I detected fatty acids by using radioactive extender-malonyl CoA.
TC
Yeah, it's good idea, I'm thinking about this ^^. Currently, we just send our samples to another lab to detect by GC, but it takes time. Our neighbor lab is cell visualization lab, maybe I can do it your way.
Hi,
did you have any luck measuring butanol? We need to measure similar levels (<1%) in growth media. I've contacted various chemistry people and have come up against a brick wall. Any assistance would be appreciated.
p
PJR on Jul 27 2009, 05:54 PM said:
did you have any luck measuring butanol? We need to measure similar levels (<1%) in growth media. I've contacted various chemistry people and have come up against a brick wall. Any assistance would be appreciated.
p
Now we just use the Gas Chromatography (GC) and it works well. It took about 2 days to have a result.