Reuse DNA spin column - (Feb/03/2009 )

Reuse DNA spin column

QBT buffer

750 mM NaCl;
50 mM MOPS,
15% isopropanol (v/v);
0.15% Triton® X-100 (v/v)
pH 7.0;

It is a nice find. I'm gonna give it a try.

Minnie Mouse on Feb 3 2009, 04:08 PM said:

Thanks Minnie mouse to post my paper (i am one of the author of this paper)

Apoptosiss on Feb 5 2009, 06:51 AM said:

Thank you for publishing this paper. You have saved our money

If you have further tips on reusing the DNA spin column, please post them here?

We would love to know

Has anyone tried reusing RNA columns such as Qiagen's RNeasy column?

The reconstitution papers referenced here fail to describe the N3 buffer used in the Qiagen minipreps. The guanidine hydrochloride used in buffers N3 and PB are not described, although these are important to binding DNA to the column. See this page for the composition of these buffers:

http://openwetware.org/wiki/Miniprep/Qiagen_kit

You might want to look at the Qiagen patent, which is linked off of that page as well.

Thank you for the link, phage434.

Welcome back phage434.

Other article for reuse DNA silica column:

Analytical Biochemistry
Volume 385, Issue 1, 1 February 2009, Pages 182-183
” Complete decontamination and regeneration of DNA purification silica columns”

thank you, labtek