Apoptosis Protocol - Abcam (Feb/01/2009 )

Introduction to apoptosis
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Annexin V detection protocol
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Apoptosis induction protocol
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Caspase detection protocol
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DNA fragmentation analysis protocol
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very helpful, thank you mm

Thanks for great links

Why can't dendritic cells proliferate? Because it is widely believed that after the formation of an IMMUNOLOGICAL SYNAPSE there is BIDIRECTIONAL SIGNALING rather that unidirectional! But still there seems to be some factor behind this that prevents DCs from proliferating? This could be true in the viewpoint of macrophages too, which also cannot divide by itself, being a nucleated cell!!! Can someone answer this? I think some CELL BIOLOGIST could pinpoint as to why DCs and Macrophages cannot proliferate upon external stimuli!!

Hi.,
Thanks for the links.
Very helpful and advantage in my part.
God Bless!



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Thank you!

I myself found that I always tend to go to the old Boehringer Mannheim Apoptosis and Cell Death manual if I have a question in this area. It is out of print for a long time, but fortunately available as PDF download from the current Roche site:

http://www.roche-applied-science.com/sis/apoptosis/docs/manual_apoptosis.pdf

What I really like about the manual is that it clearly introduces differences between apoptosis, cell death, cytotoxity, and cell proliferation and explains what tools can be used to distinguish and measure these processes. Most scientists I have met, even card-carrying cell biologists, confuse the differences between the apoptosis, cell death, and cytotoxity at least on some level so it helps to refer to this guide if you are not sure of the difference.



--
Anton
Principal Consultant, Molecular and Cell Biology

Phone: (518) 334-0922
E-mail: antonb@aleralabs.com
Web: www.aleralabs.com

Alera Labs
P.O. Box 13019
Research Triangle Park, NC 27709

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