PLASMID ISOLATION - (Jan/30/2009 )

I think I can help

Unless there is another type of pREP plasmid which I am not aware off, the pREP vector is the generic name for a family of vectors used in S. pombe (Fission yeast aka the other yeast) which are structurally similar but differ in the strength of their nmt promoter.

So we have pREP1 (strongest), pREP3, pREP41, pREP81(weakest). You will have to determine which pREP vector you have, if worst comes to worst by sequencing.
Forsburg's website


You are very right dear..
its pREP1 and i have its sequence, will follow your suggestions.
Thanks a lot for your help
regards
nipun