Fluorescence & Flow Cytometry Tutorial - (Jan/27/2009 )
I found this Flow protocol that might be useful as well. 
https://www.genetex.com/uploaddata/Protocol/Document/20140722_Immuno-fluorescent.pdf
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I found this Flow protocol that might be useful as well. 
https://www.genetex.com/uploaddata/Protocol/Document/20140722_Immuno-fluorescent.pdf