cell culture medium - (Jul/25/2021 )

HI,

Does anyone know if there are any consequences to heating RMPI medium (with 10% FBS) to 60deg for 15-20mins.

I left my culture medium in a water bath assuming the temp to be at 37deg, but after 15-20 mins I realised it was set at 60deg.

Can I use the medium?

Thank you

General rule - if in doubt, throw it out. You don't want to be wondering if your results are because of your treatment or because your medium wasn't good. 

Cell culture medium contains a number of heat-labile compounds that degrade fairly fast.