DNA ladders on a northern blot ?.. - (Dec/18/2020 )

Hello,

I was wondering if someone could tell me if this would work;

Run my total RNA sample (looking for miRNA) with a DNA ladder on a denaturing gel.
The reason I want to use a DNA ladder is that I can label it with dye and image it and my labeled probe in the Odyssey (thus avoiding the isotope issue)

My question are:
- Will the denaturing gel denature the dsDNA ladder, or do I have to heat it, then put on ice prior to adding to the gel?
- Will the DNA ladder run at the same rate (in relation to size) as my RNA?

thanks

alexsunny

alexsunny123 on Fri Dec 18 09:38:17 2020 said:

Hello,

 

I was wondering if someone could tell me if this would work;

Run my total RNA sample (looking for miRNA) with a DNA ladder on a denaturing gel.
The reason I want to use a DNA ladder is that I can label it with dye and image it and my labeled probe in the Odyssey (thus avoiding the isotope issue)
Ometv Chatroulette Omegle Bazoocam
My question are:
- Will the denaturing gel denature the dsDNA ladder, or do I have to heat it, then put on ice prior to adding to the gel?
- Will the DNA ladder run at the same rate (in relation to size) as my RNA?

 

thanks

alexsunny

thanks my issue has been fixed.