Using Biorad SDS-PAGE apparatus for CDGE - (Mar/22/2019 )

I am planning to use CDGE in one of my experiments. However, currently we dont have a DCODE Biorad for DGGE experiments. Based on the apparatus and the method for CDGE, it looks like I can employ the method using SDS-PAGE system. Is it possible? Has anyone tried this?

I need 10 mM stock solution of dNTP. So I'm assuming you just combine all four at equal amounts to create a 100 mM solution of dNTP. Then you dilute 1/10 to create a 10 mM stock of dNTP which will be suitable for PCR? Never done it this way, and I'm 99% sure this will work, however in Science, even the little things get you. Thank you

iosman987 on Fri May 1 18:00:17 2020 said:

I need 10 mM stock solution of dNTP. So I'm assuming you just combine all four at equal amounts to create a 100 mM solution of dNTP. Then you dilute 1/10 to create a 10 mM stock of dNTP which will be suitable for PCR? Never done it this way, and I'm 99% sure this will work, however in Science, even the little things get you. Thank you

thanks my issue has been fixed.