Minimum how many strains to be screened to arrive at mic50 - (Jul/24/2018 )

Hi,

We want to calculate MIC 50 for our molecule. Minimum how many strains should be screened for this, so that the data looks authentic and reliable?

Thanks

This might help - https://onlinelibrary.wiley.com/doi/full/10.1111/j.1469-0691.2006.01377.x