Papain activity calculation - (Apr/20/2018 )

Hi,

I am confused as how to calculate the activity of Papain. 

It's in lyophilized form and I want to prepare 200 µl. So, how can I calculate the activity.

I have the following information: Size - 1 u/vial and Act: 26.1 u/mgP; 57% protein

What should I dissolve it in to prepare the solution?

Thanks

Do you have an activity that you want? Or do you want activity per ul? 

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

bob1 on Fri Apr 20 21:14:04 2018 said:

Do you have an activity that you want? Or do you want activity per ul? 

 

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

Hi Bob,

I want an activity of 20 units/ml. How do I calculate that?

200 ul is 0.2 of a ml. 

For this you can use the n=c*v

n= 20 u/ml * 0.2 ml

n=4 U

bob1 on Sat Apr 21 03:42:54 2018 said:

200 ul is 0.2 of a ml. 

 

For this you can use the n=c*v

 

n= 20 u/ml * 0.2 ml

n=4 U

So, how much of the solution (example, PBS) should I add to the lyophilized powder to make it 4U? Also, is PBS is the best solution to dissolve the Papain or do you recommend dissolving it in any other solution?

Your vial contains 1 U total... so you would add 1/20th of a ml or 50 ul. You can not make a 200 ul solution with a concentration of 20 U/ml with this vial alone.