Problem with RNA bands on RNA bleach gel - (Sep/26/2017 )

Hi, 


My RNA samples were extracted from human cell lines and stored at -80C.
I used QIAGEN RNeasy mini spin kit for RNA extraction step.
Several days later I ran my RNA samples using RNA Bleach gel and got this result.
The 28S:18S ratio isn't 2:1 
Does this mean I have to extract new RNA and dump these samples? 

https://imgur.com/a/sp2iD

p.s: I have ran other RNA samples before and got good 28S:18S ratio.

      Just wondering if this batch is good enough for downstream use (qpcr).


-Pupa-

RNA Bleach gel is not a particularly rigorous method of detecting RNA quality. If you make RT PCR detect, you can have a try. And you should remember to set negative and positive controls.