We need a way to see microRNA and DNA in vitro for reverse aging - (Apr/16/2017 )

Alright, the bioelectricity method is still in debate since the bioelectricity might not drive the cell back to its stem cell state like IPSC. We can however, take a snapshot of the gene expression of the current cell and attempts to revert the cell back into its previous state as we grow older.

New plan, a hack using messenger RNA capable of transforming the cell back to its previous state. Why modify transcription factors and genes when the end result is a messenger RNA? Just target the cell's ribosome and send the artificial messenger RNA over. One problem, delivery. Deliver the correct messenger RNA into every single cell is not a currently known technology. Transfection with virus is possible but even then it is not guaranteed 100% for the cell to transform.

First, check the messenger RNA that IPSC produce. Next, spreads that messenger RNA to all the cells in the entire body to reproduce the SALK experiment. Then we tune the messenger RNA from there

So the choice is between transfecting transcription factors or messenger RNA. Transcription factors allows a gene (of multiple nucleotides) to be transcribed to messenger RNA. While creating a messenger RNA could have more freedom (you build the nucleotides yourself). I am not knowledgeable enough to know which is a better choice. A comparison between transfection success rate and desired result is needed

What would happen when you place a stem cell next to a differentiated cell?

Can you form zygote from stem cell?

Cell signaling can trigger transcription factors.

http://www.genome.jp/kegg-bin/show_pathway?map=hsa04550&show_description=show