Reversing Formaldehyde crosslinking - (Nov/22/2016 )
Hi,
I am trying to reverse formaldehyde crosslinking and cannot find a generic protocol. I did see a suggestion of using 1 ul of 5 M NaCl to 25 ul of sample and incubating at 65 degrees overnight. Is this a good way of doing the reversal?
Thanks!
-Jen-
Crosslinks between what sorts of molecules? I'm not sure that protein cross-links can be reversed, but you might be able to do DNA/protein.
-bob1-
It is protein-protein crosslinks... Isn't formaldehyde crosslinking reversible no matter what molecules were crosslinked though?
-Jen-