Formula to calculate bacterial doubling time - (Apr/18/2016 )

Hi all,

I've checked the OD600 of my bacteria culture every hour and derive the doubling time manually from plotting graph by hand. 

But I wonder for my thesis writing, I probably needed use excel and formulas. 

I came across many formulas in this case but don't really understand them.

ie.

g=1/k, where g=doubling time and k is growth rate.

The formula for growth rate is k=logXt-logX0/0.301t.

Can anyone explain the formula for k?

Thx.

I've solved it thanks.

Meg P. Anula on Mon Apr 18 16:41:15 2016 said:

I've solved it thanks.

Maybe you should show how you solved it?

For people in the future with the same problem.

I am guessing you used the standard formula using the natural number e? Or how did you calculate it?

something like this:

pito on Tue Apr 19 15:44:49 2016 said:

 

Meg P. Anula on Mon Apr 18 16:41:15 2016 said:

I've solved it thanks.

Maybe you should show how you solved it?

For people in the future with the same problem.

 

I am guessing you used the standard formula using the natural number e? Or how did you calculate it?

something like this:

 

No I stil used the same formula as I mentioned about:

g=1/k

k=logXt-logX0/0.301(t)

The problem was I forgot to change the time unit from hour to min and thought I got the wrong answer. Silly me