total protein measurement from insect body - (Jan/04/2016 )

Hello

I want to measure a total protein amount of an insect (beetle or ant)

My question is how do i separate (extract) the proteins and then quantify them:

- what lysis buffer should i use for it if i want to measure it with bradford method?

and what do you think is the best method to measure the total  amount of proteins? bradford, PCA, or something else?

thanks a lot and i would appreciate any answer

Have a great day

Something like RIPA buffer should work well. Avoid the Bradford, as it is insensitive and reagents go off quickly. I have used the BCA assay with good effect, just be aware that some detergents are not compatible with these sorts of assays, so you need to check the data-sheets for the assays to find out how much you can use.