Cloned gene in pET system not being expressed - (Aug/24/2015 )

Cool man, glad you figured out the issue.

Crazy how something small like adding an extra ingredient to the media can be the difference between nothing and everything. 

In grad school I had a similar issue...was growing a bug to purify out a native enzyme that required iron. If we supplemented the growth media with FeSO4, the bugs grew like crap and activity of the isolated protein was poor. If we supplemented growth media with Fe-EDTA instead, bugs grew 10x's better, the purified protein was highly active and preps were highly reproducible. Could have saved a year of my life had I known.