Good parafilm - (Aug/04/2015 )
phage434 on Wed Aug 5 17:57:46 2015 said:
The major problem at 10-15 days in a bag tends to be mold growth. But you may be more sterile than I am.
That is my worry as well, especially since we do have lots of fungal spores flying around.
The Parafilm M sealing film have become so successful that new products have been introduced for specific markets such as Parafilm Budding (Grafting) Tape, Parafilm Florist Stem Wrap, and Floratape. A comparison between these different Parafilm products can be understood in a very short time period.
copied from: http://www.2spi.com/catalog/supp/parafilm.php
I'm not sure about that but.. Saran wrap? That should not melt in 37. But I don't know, we don't parafilm petri dishes at all. Not in the incubator anyway, just for the fridge storage afterwards.
And if you need a petri dish that is permeable by air (I though that parafilm would seal it airtight though).. and at the same time not get a mold inside.. seems problematic.
Parafilm is permeable to gases, but, off course, the gas exchange won't be the same as in unsealed plates
http://www.parafilm.com/products#parafilm-m
El Crazy Xabi on Tue Aug 18 03:19:19 2015 said:
Parafilm is permeable to gases, but, off course, the gas exchange won't be the same as in unsealed plates
thats the one we use/have, but its not good...
I was going to offer the same advice as Trof but, unfortunately, Saran wrap is poorly permeable to oxygen. You might want to shop around, though, as some clingfilms are gas permeable
(eg. http://shprema.en.alibaba.com/product/1514201588-220490319/Top_quality_Good_gas_permeability_PE_cling_film_for_food_wrapping_keeping_fresh.html )
In the past I have used similar clingfilm with good effect when Parafilm went too pliable, sticky/gunky at 37oC and 56oC.
You might just have to give some form of clingfilm a try and see if it suits your purposes.
This Zip locker bag are very thick and it has been used for Mycobacterium tuberculosis (Obligate aerobe https://en.wikipedia.org/wiki/Obligate_aerobe) culture on plates for 14 to 30 days with no problem.
01816E
https://www.fishersci.ca/productDetails_32048
BMF on Tue Aug 18 15:31:25 2015 said:
This Zip locker bag are very thick and it has been used for Mycobacterium tuberculosis (Obligate aerobe https://en.wikipedia.org/wiki/Obligate_aerobe) culture on plates for 14 to 30 days with no problem.
01816E
Seems interesting, any idea how many plates would go in 1 bag?
I see they are 33cm x 23cm, but how much would fit in? It seems you could only fit 1 plate in?
For this Cat Number 01816E, you can put 6 petri dishes (10 cm).