protein extinction coefficient - (Jun/25/2015 )

I have a heterodimer protein. How can I calcualte its extinction coefficient based on the amino acid sequence and also through absorbance...

best

you can try this program on expasy: http://web.expasy.org/protparam/

or look at this website: http://blogs.biochem.ncsu.edu/clarklabwiki/index.php?title=Determine_extinction_coefficient_of_a_protein