Count cell bactria using haemacytometer - (Jun/02/2015 )

i need your help so bad
i get confused with my task
if i want to count cell of bacteria using haemocytometer, should i count the square in the middle too?
and when i count total cell should i use formula :

count total cell x dilution factor/ total of squre x 10.000 cell/ml
or

(0,004mm = total volume each square)

if i use 5 square >> 5 x 0,004 mm = 0,02 mm (total volume)

so >> total cell / 0,02 mm x 1000 m3(cubic)

???

please help me

It is pretty well explained here: http://en.wikipedia.org/wiki/Hemocytometer

Did you check it?

Keep in mind: not all counting chambers use the same measurements for the squares! So you do need to keep this in mind.

also check here: http://www.hemocytometer.org/2013/04/11/hemocytometer-square-size/

and here: http://celeromics.com/en/resources/docs/Articles/Cell-counting-Neubauer-chamber.pdf

If this does not help, let us know, but I think with this information you can figure it out yourself.

About the 10.000cells/ml, not sure where you get this number?

PuspasriNT on Tue Jun 2 16:17:22 2015 said:

i need your help so bad
i get confused with my task
if i want to count cell of bacteria using haemocytometer, should i count the square in the middle too?
and when i count total cell should i use formula :

count total cell x dilution factor/ total of squre x 10.000 cell/ml
or

(0,004mm = total volume each square)

if i use 5 square >> 5 x 0,004 mm = 0,02 mm (total volume)

so >> total cell / 0,02 mm x 1000 m3(cubic)

???

please help me