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IgG control for IP - (May/31/2015 )

Hi,

 

I've recently completed co-ip experiment but have been told to include IgG as control.  This was my first experience with Co-Ip and thus I wasn't familiar with the controls.

 

My co-ip was done on protein from human cancer cell lines. For IgG control, which one to I use anti- mouse, or anti-rabbit?

 

Many thanks'

-SF_HK-

Use the same type as your ip antibodies

-bob1-