Preferred assay for measuring cytotoxicity or cell proliferation - (Apr/27/2015 )

Hi all,

Please I would like to know the views of the experts here on the best method to measure cytotoxicity. I generally use MTS assay but I have come across a few papers that indicate it might not be sensitive enough and I don't want to be faulted based on that. So please experts share your views....

Cheers!

I believe MTT is still the most popular method out there.

This webinar might be useful for you. They are pushing their own products but it gives a useful overview of how to link different types of assays to get a complete picture of cytotoxicity.

< https://promega.adobeconnect.com/_a55163941/p68s9n0tu1l/?launcher=false&fcsContent=true&pbMode=normal >

btw. I use PerkinElmer's ATPLite for general cytotox screening, despite it's high price, because I find it faster and gives tighter error bars.