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taaaataaaata aaataacaca aaataaaata aaataaaata aaataaaata aaataataaa
ataaaataaa ataaaataaa ataaaataaa ataaagcaat ttcctttcct ctaagcggcc
tccacccctc tcccctgccc tgtgaagcgg gtgtgcaagc tccgggatcg cagcggtctt
agggaatttc cccccgcgat gtcccggcgc gccagttcgc tgcgcacact tcgctgcggt
cctcttcctg ctgtctgttt actccctagg ccccgctggg gacctgggaa agagggaaag
gcttccccgg ccagctgcgc ggcgactccg gggactccag ggcgcccctc tgcggccgac

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Sequence Name: GSTP1
Sequence Length: 362

CpG island prediction results
Criteria used: Island size > 100, GC Percent > 50.0, Obs/Exp > 0.60
1 CpG island(s) were found in your sequence
Size (Start - End)
Island 1 189 bp (116 - 304)

Primer picking results for methylation specific PCR (MSP)

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Primer Start Size Tm GC% 'C's Sequence
1 Left M primer 208 27 69.54 70.37 6 GGCGCGTTAGTTCGTTGCGTATATTTC
Right M primer 347 23 69.57 78.26 6 ACGCCCTAAAATCCCCGAAATCG
Product size: 140, Tm: 57.4
Left U primer 209 28 57.03 71.43 7 GTGTGTTAGTTTGTTGTGTATATTTTGT
Right U primer 347 25 65.94 80.00 6 ACACCCTAAAATCCCCAAAATCACC
Product size: 139, Tm: 57.1

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