Differences between DH5a strain of E. Coli and BL21 strain of E.Coli - (Apr/01/2015 )
Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli
-Burak Kaan Yasdı-
Burak Kaan Yasdı on Wed Apr 1 14:37:59 2015 said:
Can anybody inform me about differences between DH5a strain of E. Coli and BL21 strain of E.Coli
https://bio.as.uky.edu/sites/default/files/E_coli_host_strains_genotypes.pdf
http://openwetware.org/wiki/E._coli_genotypes
Both links can be usefull..
Its hard to tell you what the differences are that matter for you...
In short: DH5a is a general strain for cloning and blue white screening
BL21 is more for protein expression, it lacks certain proteases.
-pito-
Thanksss. This is a homework from my molecular biology laboratory class.
-Burak Kaan Yasdı-