Plate coating technique for ELISA - old school! - (Feb/05/2015 )

Hi,

I am having issues coating my ELISA plates. I have a peptide (dont know what size as the customer has not released that information), that I have tried to coat a plate with using PBS and Bicarbonate buffer. I believe it is the plate coating stage that is the issue, as I have been working through the variables and this seems like the most likely issue. Anyway - I have been chatting to someone who way back in the past would coat plates by diltuing the Ag in a buffer and incubating at 37°C until the plate was dry (i.e. all the buffer had evaporated). They would then fix the plate before continuing with the ELISA. This apparantly worked well, however they cant remember what they used to fix the plates.

Does anyone have any experience with this type of plate coating? Or have any ideas what the fixing agent may be? I am hoping to try this to see waht happens before I go down the route of carrier proteins etc....

Any help would be gratefully received!,

Cheers

Emuk

have you tried a sandwich elisa?