Different drug treating time - (Feb/05/2015 )

Hi, everyone

To determine cell viability with MTT assay, many time points (6, 12, 24, 48 hours) with drug treatment are detected.

What is meaning of different time with drug treatment? 

Thanks a lot

how quickly does the drug work... do you expect the death to be instantaneous?

I don't know how to choice the time point for testing cytotoxicity of drug.

Do i just choice the time point that i can see dose-dependent?

Can different time point represent the acute or chronic toxicity?

If you don't know these details then  you need to do a range of concentrations in a time-course experiment... that's what the time series are for.