Experiment on nutrient surplus on Jurkat cell culture - (Feb/03/2015 )

Hi,

Anyone here know how to do experiment on nutrient surplus using jurkat cell culture and what does it really mean? Please advise. Thanks in advance.

why don't you tell us what you think you should do then we can give advice for improvement (if necessary)?

I am intending to seed 0.125million cells/ml in 24 well plates cell insert. Then try to add different amount of media at different wells. So i fixed the amount of cell in the each well and vary the volume of media. The hypothesis that i am trying to test is if Jurkat cells proliferate more in the condition of surplus media. 

But my concern now is if i add more media to the well, am i like diluting the cells? So i am trying to ask if there are any proper ways of testing this hypothesis. 

Thanks :)