Help with HeLa cell cultivation - (Feb/02/2015 )
Hi to everyone,
Im doing my research project for master thesis, and I have to culture HeLa cells that we got at our uni. Since I don't have experience with HeLa cells, I need help. I planing to do the cultivation with DMEM Ham's Nutrient Mixture F-12 (Sigma Aldrich), 10%FBS, 1%Pencilin/Sreptomycin and 0.25% Trypsin. Do you have any suggestions or advice's that could help me! I would really apriciate any kind of help since I have to do it for the first time, and my professors at uni. don't have experience with HeLa cell cultivation....
Thanks in advance
_THEEA_
Hela cells are among the easiest to grow. Check the ATCC website for culture details. Having said that, also talk to the people you are getting the cells from and if possible get them to give you some training in cell culture. At the very least you should see which media they use and culture your cells in that, so as to avoid physiological changes associated with different media.
bob1 on Mon Feb 2 23:49:53 2015 said:
Hela cells are among the easiest to grow. Check the ATCC website for culture details. Having said that, also talk to the people you are getting the cells from and if possible get them to give you some training in cell culture. At the very least you should see which media they use and culture your cells in that, so as to avoid physiological changes associated with different media.
Thank You bob1, I will try to get the protocol from them, however if I use different type of DMEM media, could it be a problem for my HeLas?
Yes, it could be a problem - the physiology of the cells changes depending on which media are used, which means that gene regulation changes and hence protein levels.
Okay, thanks, If I can ask you for another advice, its about Trypsin. I have seen that TypLE Express is more convenient and less harmful to the cells, however I'm in a doubt which one to use for HeLa's. Regular Trypsin + EDTA or TrypLE Express.
Thank You :)
Either should be fine... TrypLE works fine, but I have never seen any difference between that and regular trypsin.